Real-time quantitative analysis of carbon catabolite derepression of cellulolytic genes expressed in the basidiomycete Phanerochaete chrysosporium.
Identifieur interne : 000674 ( Main/Exploration ); précédent : 000673; suivant : 000675Real-time quantitative analysis of carbon catabolite derepression of cellulolytic genes expressed in the basidiomycete Phanerochaete chrysosporium.
Auteurs : Hitoshi Suzuki [Japon] ; Kiyohiko Igarashi ; Masahiro SamejimaSource :
- Applied microbiology and biotechnology [ 0175-7598 ] ; 2008.
Descripteurs français
- KwdFr :
- Cellulose (métabolisme), Cellulose 1,4-beta-cellobiosidase (génétique), Cellulose 1,4-beta-cellobiosidase (métabolisme), Glucose (métabolisme), Phanerochaete (enzymologie), Phanerochaete (génétique), Protéines fongiques (génétique), Protéines fongiques (métabolisme), RT-PCR (MeSH), Régulation de l'expression des gènes fongiques (MeSH), Transcription génétique (MeSH).
- MESH :
- enzymologie : Phanerochaete.
- génétique : Cellulose 1,4-beta-cellobiosidase, Phanerochaete, Protéines fongiques.
- métabolisme : Cellulose, Cellulose 1,4-beta-cellobiosidase, Glucose, Protéines fongiques.
- RT-PCR, Régulation de l'expression des gènes fongiques, Transcription génétique.
English descriptors
- KwdEn :
- Cellulose (metabolism), Cellulose 1,4-beta-Cellobiosidase (genetics), Cellulose 1,4-beta-Cellobiosidase (metabolism), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression Regulation, Fungal (MeSH), Glucose (metabolism), Phanerochaete (enzymology), Phanerochaete (genetics), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Transcription, Genetic (MeSH).
- MESH :
- chemical , genetics : Cellulose 1,4-beta-Cellobiosidase, Fungal Proteins.
- chemical , metabolism : Cellulose, Cellulose 1,4-beta-Cellobiosidase, Fungal Proteins, Glucose.
- enzymology : Phanerochaete.
- genetics : Phanerochaete.
- Gene Expression Regulation, Fungal, Reverse Transcriptase Polymerase Chain Reaction, Transcription, Genetic.
Abstract
Production of cellulolytic enzymes, such as cellobiohydrolases (CBH) and cellobiose dehydrogenase (CDH), by the basidiomycete Phanerochaete chrysosporium is significantly repressed in glucose-containing media; this is known as carbon catabolite repression. We have analyzed the glucose concentration dependence of transcript numbers of the cellulolytic genes (cel6A, cel7D, and cdh) and beta-glucosidase gene (bgl3A) by means of real-time quantitative reverse transcriptase polymerase chain reaction to investigate the roll of carbon catabolite derepression in these gene expression. When the mycelium of P. chrysosporium grown in glucose culture was transferred to media containing various concentrations of glucose (0-5,000 microM), the expression levels of cel6A, cel7D, and cdh were drastically influenced by glucose, whereas no significant change was observed in bgl3A. The numbers of transcripts of cel6A, cel7D, and cdh increased exponentially during incubation for 6 h in the culture without glucose, and the rates of increase were 2.1 times per hour for cel6A transcripts and 2.7 times per hour for cel7D transcripts. Moreover, derepression of cel6A and cel7D was delayed (by 1.6 and 0.6 h, respectively) when the culture contained 50 microM glucose compared with that in the absence of glucose, suggesting that the promoter activities of cel7D and cel6A are distinct under conditions of carbon catabolite derepression.
DOI: 10.1007/s00253-008-1539-6
PubMed: 18566809
Affiliations:
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sortKey="Igarashi, Kiyohiko" sort="Igarashi, Kiyohiko" uniqKey="Igarashi K" first="Kiyohiko" last="Igarashi">Kiyohiko Igarashi</name></author><author><name sortKey="Samejima, Masahiro" sort="Samejima, Masahiro" uniqKey="Samejima M" first="Masahiro" last="Samejima">Masahiro Samejima</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18566809</idno><idno type="pmid">18566809</idno><idno type="doi">10.1007/s00253-008-1539-6</idno><idno type="wicri:Area/Main/Corpus">000688</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000688</idno><idno type="wicri:Area/Main/Curation">000688</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000688</idno><idno type="wicri:Area/Main/Exploration">000688</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Real-time quantitative analysis of carbon catabolite derepression of cellulolytic genes expressed in the basidiomycete Phanerochaete chrysosporium.</title><author><name sortKey="Suzuki, Hitoshi" sort="Suzuki, Hitoshi" uniqKey="Suzuki H" first="Hitoshi" last="Suzuki">Hitoshi Suzuki</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName></affiliation></author><author><name sortKey="Igarashi, Kiyohiko" sort="Igarashi, Kiyohiko" uniqKey="Igarashi K" first="Kiyohiko" last="Igarashi">Kiyohiko 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génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Production of cellulolytic enzymes, such as cellobiohydrolases (CBH) and cellobiose dehydrogenase (CDH), by the basidiomycete Phanerochaete chrysosporium is significantly repressed in glucose-containing media; this is known as carbon catabolite repression. We have analyzed the glucose concentration dependence of transcript numbers of the cellulolytic genes (cel6A, cel7D, and cdh) and beta-glucosidase gene (bgl3A) by means of real-time quantitative reverse transcriptase polymerase chain reaction to investigate the roll of carbon catabolite derepression in these gene expression. When the mycelium of P. chrysosporium grown in glucose culture was transferred to media containing various concentrations of glucose (0-5,000 microM), the expression levels of cel6A, cel7D, and cdh were drastically influenced by glucose, whereas no significant change was observed in bgl3A. The numbers of transcripts of cel6A, cel7D, and cdh increased exponentially during incubation for 6 h in the culture without glucose, and the rates of increase were 2.1 times per hour for cel6A transcripts and 2.7 times per hour for cel7D transcripts. Moreover, derepression of cel6A and cel7D was delayed (by 1.6 and 0.6 h, respectively) when the culture contained 50 microM glucose compared with that in the absence of glucose, suggesting that the promoter activities of cel7D and cel6A are distinct under conditions of carbon catabolite derepression.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18566809</PMID><DateCompleted><Year>2008</Year><Month>10</Month><Day>02</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0175-7598</ISSN><JournalIssue CitedMedium="Print"><Volume>80</Volume><Issue>1</Issue><PubDate><Year>2008</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Real-time quantitative analysis of carbon catabolite derepression of cellulolytic genes expressed in the basidiomycete Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>99-106</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00253-008-1539-6</ELocationID><Abstract><AbstractText>Production of cellulolytic enzymes, such as cellobiohydrolases (CBH) and cellobiose dehydrogenase (CDH), by the basidiomycete Phanerochaete chrysosporium is significantly repressed in glucose-containing media; this is known as carbon catabolite repression. We have analyzed the glucose concentration dependence of transcript numbers of the cellulolytic genes (cel6A, cel7D, and cdh) and beta-glucosidase gene (bgl3A) by means of real-time quantitative reverse transcriptase polymerase chain reaction to investigate the roll of carbon catabolite derepression in these gene expression. When the mycelium of P. chrysosporium grown in glucose culture was transferred to media containing various concentrations of glucose (0-5,000 microM), the expression levels of cel6A, cel7D, and cdh were drastically influenced by glucose, whereas no significant change was observed in bgl3A. The numbers of transcripts of cel6A, cel7D, and cdh increased exponentially during incubation for 6 h in the culture without glucose, and the rates of increase were 2.1 times per hour for cel6A transcripts and 2.7 times per hour for cel7D transcripts. Moreover, derepression of cel6A and cel7D was delayed (by 1.6 and 0.6 h, respectively) when the culture contained 50 microM glucose compared with that in the absence of glucose, suggesting that the promoter activities of cel7D and cel6A are distinct under conditions of carbon catabolite derepression.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Suzuki</LastName><ForeName>Hitoshi</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Igarashi</LastName><ForeName>Kiyohiko</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Samejima</LastName><ForeName>Masahiro</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal 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MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D043366" MajorTopicYN="N">Cellulose 1,4-beta-Cellobiosidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" 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PubStatus="medline"><Year>2008</Year><Month>10</Month><Day>3</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>6</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18566809</ArticleId><ArticleId IdType="doi">10.1007/s00253-008-1539-6</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement><orgName><li>Université de Tokyo</li></orgName></list><tree><noCountry><name sortKey="Igarashi, Kiyohiko" sort="Igarashi, Kiyohiko" uniqKey="Igarashi K" first="Kiyohiko" last="Igarashi">Kiyohiko Igarashi</name><name sortKey="Samejima, Masahiro" sort="Samejima, Masahiro" uniqKey="Samejima M" first="Masahiro" last="Samejima">Masahiro Samejima</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Suzuki, Hitoshi" sort="Suzuki, Hitoshi" uniqKey="Suzuki H" first="Hitoshi" last="Suzuki">Hitoshi Suzuki</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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